BUY TICKETS: See the Daytona 500 live!
| # | Car | Driver | Team |
| 1 | 33 | Jeffrey Earnhardt | Little Joes Autos/Curtis Key Plumbing Chevrolet |
| 2 | 51 | * Timmy Hill(i) | Spoonful of Music Foundation/Bubba Burger Chevrolet |
| 3 | 83 | * Corey LaJoie | Dustless Blasting Toyota |
| 4 | 96 | * DJ Kennington | Lordco/Castrol Toyota |
| 5 | 55 | * Reed Sorenson | Toyota |
| 6 | 23 | Joey Gase(i) | Best Home Furnishings Toyota |
| 7 | 15 | Michael Waltrip | Aaron's Toyota |
| 8 | 72 | Cole Whitt | Florida Lottery Ford |
| 9 | 37 | Chris Buescher | Cottonelle Mega Roll Chevrolet |
| 10 | 7 | * Elliott Sadler(i) | Golden Corral Chevrolet |
| 11 | 75 | * Brendan Gaughan(i) | Beard Oil Distributing Chevrolet |
| 12 | 38 | David Ragan | Camping World Ford |
| 13 | 32 | Matt DiBenedetto | EJ Wade Construction Ford |
| 14 | 34 | Landon Cassill | Love's Ford |
| 15 | 95 | Michael McDowell | K-Love Radio Chevrolet |
| 16 | 3 | Austin Dillon | DOW Chevrolet |
| 17 | 47 | AJ Allmendinger | Kroger Click List Chevrolet |
| 18 | 10 | Danica Patrick | Aspen Dental Ford |
| 19 | 1 | Jamie McMurray | Cessna McDonald's Chevrolet |
| 20 | 78 | Martin Truex Jr. | Bass Pro Shops/TRACKER BOATS Toyota |
| 21 | 13 | Ty Dillon # | GEICO Chevrolet |
| 22 | 24 | Chase Elliott | NAPA Chevrolet |
| 23 | 4 | Kevin Harvick | Jimmy John's Ford |
| 24 | 18 | Kyle Busch | M&M's Toyota |
| 25 | 27 | Paul Menard | Menards/Peak Chevrolet |
| 26 | 11 | Denny Hamlin | FedEx Express Toyota |
| 27 | 77 | Erik Jones # | 5-hour Energy Extra Strength Toyota |
| 28 | 48 | Jimmie Johnson | Lowe's Chevrolet |
| 29 | 20 | Matt Kenseth | DeWalt Toyota |
| 30 | 31 | Ryan Newman | Caterpillar Chevrolet |
| 31 | 41 | Kurt Busch | Haas Automation/Monster Energy Ford |
| 32 | 88 | Dale Earnhardt Jr. | Nationwide Chevrolet |
| 33 | 14 | Clint Bowyer | Mobil 1 Ford |
| 34 | 6 | Trevor Bayne | AdvoCare Ford |
| 35 | 17 | Ricky Stenhouse Jr. | Fastenal Ford |
| 36 | 5 | Kasey Kahne | Farmers Insurance Chevrolet |
| 37 | 19 | Daniel Suarez # | ARRIS Toyota |
| 38 | 21 | Ryan Blaney | Motorcraft/Quick Lane Tire & Auto Center Ford |
| 39 | 42 | Kyle Larson | Target Chevrolet |
| 40 | 2 | Brad Keselowski | Miller Lite Ford |
| 41 | 43 | Aric Almirola | Smithfield Foods Ford |
| 42 | 22 | Joey Logano | Shell Pennzoil Ford |