Thursday night’s Bluegreen Vacation Duel at Daytona International Speedway (7 p.m. ET on FS1, MRN, SiriusXM NASCAR Radio) will set the field and lineup for the 2021 Daytona 500 (Sunday at 2:30 p.m. ET on FOX, MRN, SiriusXM NASCAR Radio).
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Below are the lineups for each duel race.
*indicates Open team that is not locked into the Daytona 500 (only two of those will race into Sunday’s event — Ryan Preece and David Ragan made the field based on their qualifying speed Wednesday night as the top two Open teams on the speed chart)
Duel #1
| Startpot | Driver | Car Number/Team |
| 1 | Alex Bowman | No. 48 Hendrick Motorsports Chevrolet |
| 2 | Aric Almirola | No. 10 Stewart-Haas Racing Ford |
| 3 | Ricky Stenhouse Jr. | No. 47 JTG Daugherty Racing Chevrolet |
| 4 | Christopher Bell | No. 20 Joe Gibbs Racing Toyota |
| 5 | Ryan Preece | No. 37 JTG Daugherty Racing Chevrolet |
| 6 | Daniel Suarez | No. 99 Trackhouse Racing Team Chevrolet |
| 7 | Denny Hamlin | No. 11 Joe Gibbs Racing Toyota |
| 8 | Kyle Larson | No. 5 Hendrick Motorsports Chevrolet |
| 9 | Matt DiBenedetto | No. 21 Wood Brothers Racing Ford |
| 10 | Austin Cindric* | No. 33 Team Penske Ford |
| 11 | Joey Logano | No. 22 Team Penske Ford |
| 12 | Ryan Newman | No. 6 Roush Fenway Racing Ford |
| 13 | Cole Custer | No. 41 Stewart-Haas Racing Ford |
| 14 | Michael McDowell | No. 34 Front Row Motorsports Ford |
| 15 | Erik Jones | No. 43 Richard Petty Motorsports Chevrolet |
| 16 | Tyler Reddick | No. 8 Richard Childress Racing Chevrolet |
| 17 | Ty Dillon* | No. 96 Gaunt Brothers Racing Toyota |
| 18 | Jamie McMurray | No. 77 Spire Motorsports Chevrolet |
| 19 | Quin Houff | No. 00 StarCom Racing Chevrolet |
| 20 | Timmy Hill* | No. 66 Motorsports Business Management Ford |
| 21 | Cody Ware | No. 51 Rick Ware Racing Chevrolet |
| 22 | Josh Bilicki | No. 52 Rick Ware Racing Ford |
Duel #2
| Start |
Driver | Team/Car Number |
| 1 | William Byron | No. 24 Hendrick Motorsports Chevrolet |
| 2 | Bubba Wallace | No. 23 23XI Racing Toyota |
| 3 | Kevin Harvick | No. 4 Stewart-Haas Racing Ford |
| 4 | Austin Dillon | No. 3 Richard Childress Racing |
| 5 | Chase Elliott | No. 9 Hendrick Motorsports Chevrolet |
| 6 | David Ragan | No. 36 Front Row Motorsports Ford |
| 7 | Kurt Busch | No. 1 Chip Ganassi Racing Chevrolet |
| 8 | Kyle Busch | No. 18 Joe Gibbs Racing Toyota |
| 9 | Ryan Blaney | No. 12 Team Penske Ford |
| 10 | Kaz Grala* | No. 16 Kaulig Racing Chevrolet |
| 11 | Chase Briscoe | No. 14 Stewart-Haas Racing Ford |
| 12 | Ross Chastain | No. 42 Chip Ganassi Racing Chevrolet |
| 13 | Brad Keselowski | No. 2 Team Penske Ford |
| 14 | Martin Truex Jr. | No. 19 Joe Gibbs Racing Toyota |
| 15 | Chris Buescher | No. 17 Roush Fenway Racing Ford |
| 16 | Anthony Alfredo | No. 38 Front Row Motorsports Ford |
| 17 | Corey LaJoie | No. 7 Spire Motorsports Chevrolet |
| 18 | Garrett Smithley* | No. 13 Motorsports Business Management Ford |
| 19 | BJ McLeod | No. 78 Live Fast Motorsports Ford |
| 20 | Joey Gase | No. 53 Rick Ware Racing Ford |
| 21 | Derrike Cope | No. 15 Rick Ware Racing Chevrolet |
| 22 | Noah Gragson* | No. 62 Beard Motorsports Chevrolet |